(3β,5β)-3-[(4-Nitrobenzoyl)oxy]-cholan-24-oic Acid is an intermediate in the synthesis of (3β,5β)-3-Hydroxycholan-24-oic Acid (H824865), which has been used for the study of the activation of β1 subunit-containing BK channels with respect to the structure of monohydroxylated bile acids.