2’,3’,5’-Tri-O-acetyl-2N,2N-dimethyl Guanosine-D6 is an intermediate used in the synthesis of 2-(Dimethylamino)guanosine-d6 (D460852), which is deuterium labeled 2-(Dimethylamino)guanosine (D460850), that can be used to investigate the mechanism of2'-deoxyoxanosine formation from 2'-deoxyguanosine and nitrous acid. In addition, 2-(Dimethylamino)guanosine can also be employed in mass spectrometry to quantify tRNA modifications related to cellular stress response inSaccharomyces cerevisiae.